Mustafa Soyöz
Burcu Akman
İsmail Totur
Sinem Topçu
Aslı Özkızılcık Koçyiğit
Burcu Çerçi Gürbüz
Ceren Yüksel
Toprak Hamdi Güngör
Ayşe Memon
Derya Rasime Güleç
Murat Kılıçoğlu
Tülay Kılıçaslan Ayna
İbrahim Pirim

Abstract

Objectives:

This study aims to investigate human leukocyte antigen (HLA) allele frequencies of healthy individuals and to determine whether there is an association between the HLA alleles and ABO blood groups.

Materials and methods:

The HLA-A, HLA-B and HLA-DRB1 allele frequency were evaluated in a sample of 450 unrelated individuals at Izmir Tepecik Education and Research Hospital Tissue Typing Laboratory archives. The HLA genotypes of individuals were analyzed by using single-specific primer- polymerase chain reaction (PCR-SSP) or PCR using sequence-specific oligonucleotide (PCR-SSO) methods. Blood groups were evaluated by using microplate agglutination method.

Results:

The most common alleles were HLA-A*02, HLA-B*35, and HLA-DRB1*11 in each locus. A and O blood groups were most common, respectively. A statistically significant association between HLA and ABO blood groups was found in HLA-B and HLA-DRB1 loci.

Conclusion:

Our study results confirm the other study findings in the Turkish population and also contribute an additional data for further HLA polymorphisms and anthropological studies. We believe that gaining a better understanding on the HLA allele frequencies would help to constitute national transplantation strategies.

Keywords:

Allele, blood type, human leukocyte antigen

VOLUME

4

,

ISSUE

1
April 2016

Correspondence

Mustafa Soyöz

Email

mustafa.soyoz@ikc.edu.tr

Received

Accepted

Published

Suggested Citation

DOI

License

This work is licensed under the Creative Commons Attribution-NonCommercial-Non-Derivatives 4.0 International License (CC BY-NC-ND 4.0). License