Turk J Immunol. 2015; 3(3): 111-116 | DOI: 10.5606/tji.2015.393

Significance of EMSA Eritin Administration on Erythropoiesis and Complement Regulators in Irradiated Mice

Qonitatul Khasanah¹, Mansur Ýbrahim², Muhamib Rifai^1
1Department of Biology, Faculty of Mathematics and Natural Sciences, University of Brawijaya, Malang, Indonesia
²Department of Biomedical Sciences, Faculty of Medicine, Brawijaya University, Malang, Indonesia

Objectives: This study aims to examine the effect of polyherbal erythropoiesis modulatory and stimulatory agent (EMSA) Eritin administration in modulating the activity of erythropoiesis on TER- 119+VLA-4+ cells and complement regulators TER-119+CD55+ a nd T ER-119+CD59+ cells in Balb/c mice after total body irradiation (TBI) with a dose of 500 rad. Materials and methods: EMSA Eritin was administered orally in mice after exposure to TBI with a dose of 500 rad daily for two weeks with three different doses including a low dose (1.04 mg/g BW), normal dose (3.125 mg/g BW), and high dose (9.375 mg/g BW). Mice were treated with HEMAPO Epoetin alfa twice a week as positive control with a dose of 0.21 mg/g BW. On day 15, the bone marrow was isolated and the number of TER-119+VLA-4+, TER-119+CD55+ a nd T ER-119+CD59+ cells were a nalyzed using flow cytometry. Examination showed marked reduction in the number of TER-119+VLA-4+ cells in the bone marrow of mice after TBI with dose of 500 rad compared to those of healthy controls. The number of TER-119+VLA-4+ cells in the i rradiated m ice was markedly increased with EMSA Eritin from low dose to high dose compared to mice with TBI and mice treated with Epoetin alfa. Results: The highest dose of EMSA Eritin was more effective to promote erythropoiesis than HEMAPO Epoetin alfa and the other dose of EMSA Eritin. Conclusion: Our study findings suggest that EMSA Eritin is a powerful medicinal herbal supplement which may serve as a protective agent to normalize homeostasis and erythropoiesis after irradiation with a dose of 500 rad.

Keywords: Antioxidant; bone marrow; complement regulators; erythropoiesis; total body irradiation.

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Radyasyona Maruz Kalan Farelerde EMSA Eritin Uygulamasýnýn Eritropoez ve Kompleman Regülatörler Üzerindeki Önemi

Qonitatul Khasanah¹, Mansur Ýbrahim², Muhamib Rifai^1
1Department of Biology, Faculty of Mathematics and Natural Sciences, University of Brawijaya, Malang, Indonesia
²Department of Biomedical Sciences, Faculty of Medicine, Brawijaya University, Malang, Indonesia

Amaç: Bu çalýþmada Balb/c farelerde 500 rad doz ile total vücut irradyasyonu (TVÝ) sonrasý poliherbal eritropoez modülatör ve stimülatör ajan (EMSA) Eritin uygulamasýnýn TER-119+VLA-4+ hücrelerine göre eritropoez aktivitesi ve TER-119+CD55+ ile TER-119+CD59+ hücrelerine göre kompleman regülatörleri modülasyonu üzerindeki etkisi incelendi. Gereç ve yöntemler: Düþük doz (1.04 mg/g BW), normal doz (3.125 mg/g BW) ve yüksek doz (9.375 mg/g BW) içerecek þekilde iki hafta boyunca günde 500 rad dozunda TVÝ’ye maruz kalan farelere oral yoldan EMSA Eritin uygulandý. Fareler pozitif kontrol olarak haftada iki kere 0.21 mg/g BW dozunda HEMAPO Epoetin alfa ile tedavi edildi. On beþinci günde kemik iliði izole edildi ve akýþ sitometrisi kullanýlarak TER-119+VLA-4+, TER-119+CD55+ v e T ER-119+CD59+ hücrelerin sayýsý analiz edildi. Ýnceleme, 500 rad dozunda TVÝ sonrasý farelerin kemik iliðindeki TER-119+VLA-4+ hücrelerin sayýsýnda saðlýklý kontrollere göre anlamlý azalma olduðunu gösterdi. Radyasyona maruz kalan farelerde EMSA Eritin ile TER-119+VLA-4+ hücrelerin sayýsý TVÝ olan fareler ve Epoetin alfa ile tedavi edilen farelere göre düþük dozdan yüksek doza anlamlý þekilde arttý. Bulgular: En yüksek dozda EMSA Eritin eritropoezi desteklemede HEMAPO Epoetin alfa ve diðer dozdaki EMSA Eritin’e göre daha etkili idi. Sonuç: Çalýþma bulgularýmýza göre, EMSA Eritin 500 rad doz ile irradyasyon sonrasý homeostaz ve eritropoezi normalleþtiren koruyucu bir ajan iþlevi gösterebilecek güçlü bir tedavici edici herbal destekleyicidir.

Anahtar Kelimeler: Antioksidan; kemik iliði; kompleman regülatörler; eritropoez; total vücut irradyasyonu

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