Turk J Immunol. 2020; 8(2): 44-49 | DOI: 10.25002/tji.2020.1242

Impact of Storage Period on CD4+/CD8+ T Lymphocyte Ratio in Erythrocyte Suspensions

Salih Haldun Bal¹, Levent Tufan Kumaþ¹, Yasemin Heper¹, Ferah Budak², Gülher Göral³, Fatma Ezgi Can⁴, Haluk Barbaros Oral^1
1Bursa Uludag University, Faculty of Medicine, Dr. Raþit Durusoy Kan Merkezi, Bursa, Turkey
²Bursa Uludag University, Faculty of Medicine, Department of Immunology, Bursa, Turkey
³Bursa Uludag University, Faculty of Medicine, Department of Medical Microbiology, Bursa, Turkey
⁴Ýzmir Katip Celebi University, Faculty of Medicine, Department of Biostatistics, Ýzmir, Turkey

Introduction: Some immunologic changes in the recipient derived by allogeneic blood transfusion (ABT) are called Transfusion Related Immune Modulation (TRIM). Despite the exact mechanisms of these changes are not known, it is thought that ABT causes a decrease of CD4/CD8 ratio in the recipient. This study aimed to determine the CD4/CD8 ratio in stored erythrocyte suspensions (ES) and to obtain new information about TRIM mechanisms. Materials and Methods: Whole blood components used in our study were collected from 10 healthy volunteers. ES’ obtained from whole blood were divided into three equal aliquots. Test samples which were related to 0th, 21st and 42nd storage days were prepared from these aliquots. CD3, CD4 and CD8 surface markers in peripheral blood mononuclear cells were investigated with flow-cytometer in these test samples. Results: Our data were evaluated according to storage days. Decrease of CD3 (p=0,001), CD4 (p<0,001), CD8 (p=0,012) expressing mononuclear blood cells and helper T cells in 21st-day samples, CD4 (p=0,035) and CD8 (p=0,017) expressing cells in 42nd-day samples compared to Day 0 samples and increase of CD3 (p=0,027) expressing cells in 42nd-day samples compared to 21st-day samples were found statistically significant. Conclusion: In our study, we did not find any significant change in the ratio of CD4+/CD8+ cells in ES. Detailed studies can help us to obtain more comprehensive knowledge on this field.

Keywords: CD4-CD8 ratio, erythrocyte suspension, erythrocyte transfusion, immunomodulation, transfusion

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Eritrosit Süspansiyonlarýnda Depolanma Sürecinin CD4+/CD8+ T Lenfositleri Oraný Üzerine Etkisi

Salih Haldun Bal¹, Levent Tufan Kumaþ¹, Yasemin Heper¹, Ferah Budak², Gülher Göral³, Fatma Ezgi Can⁴, Haluk Barbaros Oral^1
1Bursa Uludag University, Faculty of Medicine, Dr. Raþit Durusoy Kan Merkezi, Bursa, Turkey
²Bursa Uludag University, Faculty of Medicine, Department of Immunology, Bursa, Turkey
³Bursa Uludag University, Faculty of Medicine, Department of Medical Microbiology, Bursa, Turkey
⁴Ýzmir Katip Celebi University, Faculty of Medicine, Department of Biostatistics, Ýzmir, Turkey

Giriþ: Allojeneik kan transfüzyonunun (AKT) alýcýda neden olduðu bazý immünolojik deðiþikliklere transfüzyonla iliþkili immunomodülasyon (TRIM) adý verilmektedir. Bu deðiþikliklerin mekanizmasý tam olarak bilinmese de, AKT’nin alýcýnýn mononülear kan hücrelerindeki CD4/CD8 ifadesi oranýnda azalmaya yol açtýðýna inanýlmaktadýr. Bu çalýþmada depolanan eritrosit süspansiyonlarýndan elde edilen mononükleer hücrelerdeki (ES) CD4/CD8 ifadesi oranýný belirlemek ve TRIM mekanizmalarýna yönelik yeni bilgiler edinmek amaçlanmýþtýr. Gereç ve Yöntemler: Çalýþmamýzda kullanýlan tam kanlar saðlýklý 10 gönüllüden elde edilmiþtir. Bu tam kanlardan elde edilen ES’ler eþit üç parçaya bölünmüþ ve ES’lerin 0, 21 ve 42. raf günlerine yönelik test örnekleri bu parçalardan elde edilmiþtir. Test örneklerdeki CD3, CD4 ve CD8 yüzey belirteçleri akan hücre ölçer yardýmýyla incelenmiþtir. Bulgular: Verilerimiz ES’lerin depolanma günlerine gore deðerlendirilmiþtir. Sýfýrýncý gün örneklerine göre 21. gün örneklerinde CD3 (p=0,001), CD4 (p<0,001), CD8 (p=0,012) ifade eden hücrelerde ve yardýmcý T hücrelerinde görülen azalma (p<0,001); 42. gün örneklerinde CD4 (p=0,035) ve CD8 (p=0,017) eksprese eden hücrelerde görülen azalma; 21. gün örneklerine göre 42. gün örneklerinde CD3 (p=0,027) ifade eden hücrelerde görülen artýþ istatistiksel olarak anlamlý bulunmuþtur. Sonuç: Çalýþmamýzda ES’ler içerisinde CD4+/CD8+ hücre oranýnda istatistiksel olarak anlamlý bir deðiþiklik saptanmamýþtýr. Bu konuda daha kapsamlý bilgiye sahip olmak için daha detaylý çalýþmalara ihtiyaç vardýr.

Anahtar Kelimeler: CD4-CD8 oraný, eritrosit süspansiyonu, eritrosit transfüzyonu, immunomodülasyon, transfüzyon

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