Turk J Immunol. 2018; 6(1): 16-22 | DOI: 10.25002/tji.2018.722

The Relationship Between Serum Vitamin D (25(OH)D3), C-Peptide, and Interleukin-2 Level in Children with Type 1 Diabetes Mellitus

Harjoedi Adji Tjahjono¹, Vivi Ratnasari², Edi Widjajanto³, Andreas Budi Wijaya^4
1Saiful Anwar General Hospital, Department of Pediatrics, Malang/ East Java, Indonesia
²Brawijaya University, Biomedical Science Master Study Program, Malang/East Java, Indonesia
³Saiful Anwar General Hospital, Department of Clinical Pathology, Malang/East Java, Indonesia
⁴Brawijaya University, Faculty of Medicine, Malang/East Java, Indonesia

Introduction: Vitamin D is very important for Type 1 diabetes mellitus (T1 DM) pathogenesis, and plays major role in the regulation of pancreatic beta cells. However, serum vitamin D (25(OH)D3) level related to inflammatory condition in T1 DM has not been sufficiently investigated. Therefore, we aimed to investigate the relationship between serum vitamin D (25(OH)D3), C-Peptide, and Interleukin-2 levels in children with T1 DM. Material and Methods: Blood samples from 20 subjects of T1 DM patients and healthy patients were collected, and analyzed. Serum level of vitamin D (25(OH)D3), C-Peptide, and Interleukin-2 between two groups were measured by using Indirect ELISA. Results: Serum vitamin D (25(OH)D3), C-Peptide, and Interleukin-2 level between both groups were significantly different (p<0.001). It was shown that serum vitamin D (25(OH)D3) and C-Peptide level had significant positive correlation, while serum C-Peptide and Interleukin-2 level had significant negative correlation (p=0.002; r=-0.658). Serum vitamin D (25(OH)D3) and Interleukin-2 had also significant negative correlation (p<0.001; r=-0.753). Discussion: Serum vitamin D (25(OH)D3) level is related to inflammatory condition in T1 DM. Low level of serum vitamin D (25(OH)D3) in T1 DM patient tends to increase the level of Inteleukin-2, and it is highly correlated with pancreatic beta cells’ impairment, which is marked by low C-Peptide level and high Inteleukin-2 level.

Keywords: C-Peptide, interleukin-2, T1 DM, vitamin D (25(OH)D3)

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Tip 1 Diabetes Mellitus’lu Çocuklarda Serum D Vitamini (25(OH)D3), C-Peptid ve Ýnterlökin-2 Seviyeleri Arasýndaki Ýliþki

Harjoedi Adji Tjahjono¹, Vivi Ratnasari², Edi Widjajanto³, Andreas Budi Wijaya^4
1Saiful Anwar General Hospital, Department of Pediatrics, Malang/ East Java, Indonesia
²Brawijaya University, Biomedical Science Master Study Program, Malang/East Java, Indonesia
³Saiful Anwar General Hospital, Department of Clinical Pathology, Malang/East Java, Indonesia
⁴Brawijaya University, Faculty of Medicine, Malang/East Java, Indonesia

Giriþ: D vitamini, tip 1 diabetes mellitus (T1 DM) oluþumunda pankeasta beta hücre düzenlenmesinde çok önemlidir ve pankreastaki beta hücrelerini düzenler. Ancak, serumdaki D vitamini (25(OH)D3) seviyesinin T1 DM hastalarýnda, inflamatuvar durum ile iliþkisi yeterli düzeyde araþtýrýlmamýþtýr. Bu nedenle, çalýþmamýzda T1 DM tanýsý alan çocuklarda serum D vitamini, C-peptid ve Ýnterlökin-2 düzeyleri arasýndaki iliþkinin saptanmasýý amaçlanmýþtýr. Gereçler ve Yöntemler: T1 DM olan 20 hastanýn ve saðlýklý bireylerin kan örneklerini alarak irdeledik. Serum örneklerinde D vitamini (25(OH)D3), C-Peptid ve Ýnterlökin-2 düzeyleri inderekt ELISA tetsi ile ölçüldü. Bulgular: Her iki gruptaki serum D vitamini (25(OH)D3), C-peptid ve Ýnterlökin-2 düzeyleri anlamlý düzeyde birbirinden farklýlýk gösterdi (p<0.001). Serum D vitamini (25(OH)D3) ve C-peptid seviyeleri arasýnda doðrusal bir iliþki saptanmasýna karþýlýk, C-peptid ve Ýnterlökin-2 düzeyleri arasýnda ters bir baðlantý saptanmýþtýr (p=0.002; r=- 0.658). Ayrýca Serum D vitamini (25(OH)D3) ve Ýnterlökin-2 düzeyleri arasýnda da ters bir baðlantý saptanmýþtýr (p<0.001; r=-0.753). Sonuç: Serumdaki D vitamini (25(OH)D3) düzeyi, T1 DM’deki inflamatuvar durum ile iliþkilidir. D vitamininin düþük olmasý T1 DM’lu hastalarda Ýnterlökin-2 düzeyinin artýþý ile baðýntýlý gibi gözükmektedir ve pankreastaki beta hücrelerinin yetersizliðini gösteren düþük C-peptid ve yüksek Interleukin-2 düzeyi ile önemli ölçüde iliþkilidir.

Anahtar Kelimeler: C-Peptid, Ýnterlökin-2, Tip 1 Diabetes Mellitus, D Vitamini (25(OH)D3)

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