ISSN 1301-109X | e-ISSN 2147-8325
Accurate and Simple Interpretation of HLA B27 Screening by Flow Cytometry [Turk J Immunol]
Turk J Immunol. 2016; 4(1): 1-6 | DOI: 10.5606/tji.2016.459

Accurate and Simple Interpretation of HLA B27 Screening by Flow Cytometry

Gülderen Yanıkkaya Demirel1, Ömer Güzel2
1Department of Immunology, Medical Faculty of Yeditepe University, İstanbul, Turkey
2Centro Laboratuvarları, İstanbul, Turkey

Objectives: This study aims to compare the results of the human leukocyte antigen (HLA) B27 screenings by flow cytometry with results obtained by the sequence-specific primer-polymerase chain reaction (SSP-PCR) HLA B27 analysis.
Patients and methods: B etween J anuary 0 1st, 2008 and December 31st, 2008, the flow cytometry screening results of HLA B27 analysis for 486 samples were retrospectively analyzed. The results were compared with those obtained by the SSP-PCR for the diagnostic accuracy.
Results: The sensitivity of HLA B27 screening test by flow cytometry was 99% while the specificity was 100%. For our study population, while positive predictive value was 0.99 for HLA B27 screening for cytometry; negative predictive value was 1. HLA B7 positivity is detected in 0.8% of all patients.
Conclusion: Our study results suggest that accurate flow cytometric screening test for HLA B27 is a reliable screening test with high specificity and sensitivity and can be safely used by the clinicians.

Keywords: Ankylosing spondylitis, cytometry, HLA B27

Corresponding Author: Gülderen Yanıkkaya Demirel, Türkiye
Manuscript Language: English
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