Turk J Immunol. 2022; 10(3): 170-178 | DOI: 10.4274/tji.galenos.2022.54154

Evaluating the Effect of a Food-origin Lactobacillus plantarum Strain on Th17 Related Cytokines

Mehri Joghataei¹, Gholamreza Pouladfar², Fakhri Shahidi¹, Mehdi Kalani^2
1Ferdowsi University of Mashhad, Faculty of Agriculture, Department of Food Science and Technology, Mashhad, Iran
²Professor Alborzi Clinical Microbiology Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, Iran

Objective: This study aimed to assess the impact of a wild strain of Lactobacillus plantarum (PL4) on the production of IL-10 and T helper (Th) 17 related cytokines, including interleukin (IL)-17A, IL-17F, IL-21, and IL-22, compared to a standard probiotic strain, Lactobacillus plantarum. PL4 was isolated from Lighvan cheese and its probiotic potential was proved before. Probiotics with immune regulatory activity might be effective in the case of Th17 cells and IL-17-related pathways, which are involved in the pathogenesis and are associated with a worse result of some diseases. These regulatory effects might be arising from different bacterial cell substances.
Materials and Methods: Peripheral blood mononuclear cells (PBMC) were treated with cell debris and cell extract of both strains with and without human anti-CD3/CD28 T-cell activator beads in order to determine immunomodulatory effects of the strains. A number of cytokines were assessed.
Results: Bacterial cell debris and extract had no stimulatory or suppressive effects on the Th17 related cytokines and only the debris of both strains increased IL-10 production significantly. When PBMC pretreated with bacterial cell debris or extracts was stimulated with anti-CD3/CD28 beads, both strains down-regulated IL-17F. PL4 extract could decrease IL-21 and IL-17A but conversely, its debris increased these cytokines.
Conclusion: Pretreatment with PL4 extract induced lower levels of IL-17A, IL-17F, IL-21 and IL-10 in stimulated PBMCs, implying an antiinflammatory potential of this strain. Significant differences among the effects of the strains confirmed strain dependency of their immunomodulatory properties.

Keywords: Lactobacillus plantarum, immunomodulatory properties, Th17 related cytokines, PBMC

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Gýda Kökenli Lactobacillus plantarum Suþunun Th17 ile Ýliþkili Sitokinler Üzerindeki Etkisinin Deðerlendirilmesi

Mehri Joghataei¹, Gholamreza Pouladfar², Fakhri Shahidi¹, Mehdi Kalani^2
1Ferdowsi University of Mashhad, Faculty of Agriculture, Department of Food Science and Technology, Mashhad, Iran
²Professor Alborzi Clinical Microbiology Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, Iran

Amaç: Bu çalýþmada, yabani bir Lactobacillus plantarum (PL4) suþunun, interlökin (IL)-10 ve IL-17A, IL-17F, IL-21 ve IL-22 dahil olmak üzere T helper (Th) 17 ile iliþkili sitokinlerin üretimi üzerindeki etkisini, standart bir probiyotik suþ olan Lactobacillus plantarum ile karþýlaþtýrarak deðerlendirmesi amaçlanmýþtýr. PL4, Lighvan peynirinden izole edilmiþ ve probiyotik potansiyeli daha önce kanýtlanmýþtýr. Ýmmün düzenleyici aktiviteye sahip probiyotikler, patogenezde yer alan ve bazý hastalýklarýn daha kötü bir sonucu ile iliþkilendirilen Th17 hücreleri ve IL-17 ile ilgili yolaklar durumunda etkili olabilmekte ve bu düzenleyici etkiler, farklý bakteri hücre maddelerinden kaynaklanabilmektedir.
Gereç ve Yöntem: Periferik kan mononükleer hücreleri (PBMC), suþlarýn immünomodülatör etkilerini belirlemek için, insan anti-CD3/CD28 T hücre aktivatör boncuklarý olan ve olmayan her iki suþun hücre debrisi ve hücre ekstraktý ile muamele edildi. Kültür süpernatanlarýnda sitokinlerin miktarlarý belirlendi.
Bulgular: Bakteriyel hücre debris ve ekstraktý, Th17 ile ilgili sitokinler üzerinde hiçbir uyarýcý veya baskýlayýcý etki göstermedi ve sadece her iki suþun debrisi IL-10 üretimini anlamlý ölçüde artýrdý. Bakteriyel hücre debrisi veya ekstraktý ile ön iþleme tabi tutulan PBMC, anti-CD3/CD28 boncuklarý ile uyarýldýðýnda, her iki suþ da IL-17F seviyelerini azalttý. PL4 ekstraktý, IL-21 ve IL-17A seviyelerini azaltmasýnýn tersine PL4 ekstrat debrisi bu sitokinleri artýrdý.
Sonuç: PL4 ekstraktý ile ön muamele, uyarýlmýþ PBMC’lerde daha düþük IL-17A, IL-17F, IL-21 ve IL-10 seviyelerine neden olmuþtur, bu bulgular bu suþun anti-enflamatuvar potansiyelini göstermektedir. Suþlarýn etkileri arasýndaki önemli farklýlýklar, immünomodülatör özelliklerinin suþ baðýmlýlýðýný doðrulamaktadýr.

Anahtar Kelimeler: Lactobacillus plantarum, immunomodulatory properties, Th17 related cytokines, PBMC

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