Turk J Immunol. 2017; 5(2): 51-60 | DOI: 10.25002/tji.2017.539

The Effect of Lactobacillus reuteri on the Percentage of Th17 Cells and Level of IL-17 in Staphylococcus aureus- Induced Puerperal Infection BALB/c Mice

Uswatun Hasanah¹, Sitti Candra Windu Baktiyani², Sri Poeranto³, Tatit Nurseta², Sumarno Reto Prawiro^4
1Midwifery Department Faculty of Medicine University of Brawijaya, Malang, Indonesia
²Gynecological Obstetrics Laboratory Faculty of Medicine University of Brawijaya, Malang, Indonesia
³Parasitology Laboratory, Faculty of Medicine University of Brawijaya, Malang, Indonesia
⁴Microbiology Laboratory, Faculty of Medicine University of Brawijaya, Malang, Indonesia

Background: Puerperal infection is an infection of the reproductive tract during labor or puerperal period, which is largely caused by Staphylococcus aureus infection resulting in Toxic Shock Syndrome (TSS). The prevalence of postpartum infection has increased over the past three years, coupled with an increase in Staphylococcus aureus (MRSA) Methicillin Resistant, resulting in high treatment costs and high morbidity and mortality rates. Objective: This study aimed to determine the effect of Lactobacillus reuteri on the percentage of Th17 cells and the level of IL-17 in Staphylococcus aureus-induced puerperal BALB/c mice. Methods: Mice were divided into 4 groups with each group consisting of 4 mice in puerperal period and 4 mice in three days postpartum period; Group I (mice were induced with Staphylococcus aureus at 0–12 hours postpartum), Group II (mice were administered orally with Lactobacillus reuteri), Group III (mice were treated with Lactobacillus reuteri and Staphylococcus aureus) and group IV (control). The percentage of Th17 cells was measured by Flow cytometry method, while the level of IL-17 was measured by ELISA method. Results: The results showed that the administration of Lactobacillus reuteri significantly influenced the percentage of Th17 cells and the levels of IL-17 in Staphylococcus aureus-induced puerperal mice. Conclusion: In summary, Lactobacillus reuteri may act as a preventive agent of puerperal infections in Staphylococcus aureus-induced mice during the puerperal period and three days postpartum.

Keywords: IL-17, Lactobacillus reuteri, Staphylococcus aureus, Th17 cells

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Farelerde Staphycoccus aureus ile Oluþturulan Lohusalýk Ateþinde Lactobacillus reuteri’nin Th17 Hücreleri ve IL-17 Düzeyine Olan Etkisi

Uswatun Hasanah¹, Sitti Candra Windu Baktiyani², Sri Poeranto³, Tatit Nurseta², Sumarno Reto Prawiro^4
1Midwifery Department Faculty of Medicine University of Brawijaya, Malang, Indonesia
²Gynecological Obstetrics Laboratory Faculty of Medicine University of Brawijaya, Malang, Indonesia
³Parasitology Laboratory, Faculty of Medicine University of Brawijaya, Malang, Indonesia
⁴Microbiology Laboratory, Faculty of Medicine University of Brawijaya, Malang, Indonesia

Giriþ: Lohusalýk ateþi doðum sýrasýnda veya lohusalýk dönem Staphilococcus aureus enfeksiyonunun neden olduðu, toksik þok sendromuna (TSS) neden olan bir üreme sistemi enfeksiyonudur. Son 3 yýldýr metisiline dirençlý Staphylococcus aureusun neden olduðu doðum sonrasý enfeksiyonlarda artýþ olmuþtur. Bu artýþ ta tedavi maliyetlerinin morbidite ve mortalite oranýnda artýþ olmuþtur. Amaç: Bu çalýþmada amaç Balb/c farelerde Staphylococcus aureus ile oluþturulmuþ lohusalýk ateþinde Lactobacillus reuteri’nin Th17’nin ve Il-17 seviyelerine olan etkisi araþtýrmaktýr. Yöntemler: Bu çalýþmada, her grupta tam lohusalýk döneminde olan 4 fare ile doðumdan 3 gün sonra gruplandýrýlmýþ 4 fare bulunduðu 4 fare bulunmakta idi: Grup I (Doðumdan 0-12 saat sonra Staphylococcus aureus verilmiþ fareler), Grup II (sadece aðýzdan Lactobacillus reuteri verilmiþ fareler), Grup III (Lactobacillus reuteri ve Staphylococcus aureus verilmiþ fareler), Grup IV (Kontrol). Akan hücre ölçer ile Th17 hücrelerinin oraný ve ELISA yöntemi ile IL-17 ölçüldü. Sunular: Çalýþmada, Staphylococcus aureus ile oluþturulmuþ lohusalýk ateþi olan farelerde Lactobacillus reuteri verilmesinin Th17 hücrelerinin oranýný ve IL-17 seviyesini düþürdüðü saptandý. Sonuç: Lactobacillus reuteri, Staphylococcus aureus ile doðum döneminde ve doðumdan 3 gün sonra oluþturulmuþ lohusalýk ateþinde koruyucu bir rol oynamaktadýr.

Anahtar Kelimeler: IL-17, Lactobacillus reuteri, Staphilococcus aureus, Th17 hücreleri

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