Turk J Immunol. 2017; 5(2): 31-35 | DOI: 10.25002/tji.2017.550

The Associations of Plasma Levels of Beta Amyloid, Insulin, Insulin-Degrading Enzyme and Receptor of Advanced Glycosylation End Product with Cognitive Impairment in Type 2 Diabetes Mellitus Patients

Yuliarni Syarita¹, Darwin Amir¹, Eva Decroli¹
Faculty of Medicine, Andalas University/DR M Djamil Hospital, Jalan Perintis Kemerdekaan No.94 Padang Timur, Padang, West Sumatra, Indonesia, Department of Neurology, Padang, Indonesia

Background: Diabetes mellitus patients are at a higher risk of developing cognitive impairment compared to the nondiabetes mellitus population. There are various mechanisms underlying the association between diabetes mellitus and impaired cognitive function, including the failure of glucose metabolism, insulin resistance, and increased formation of amyloid beta, insulin-degrading enzyme (IDE) and receptor of advanced glycosylation end product (RAGE). Objective: This study aimed to find the association of the levels of beta amyloid, insulin, IDE, and RAGE with the onset of cognitive impairment in diabetes mellitus patients. Methods: A case control study was conducted on 60 patients classified as 30 subjects with cognitive impairment and 30 subjects without cognitive impairment. Cognitive function was examined using the Montreal Cognitive Assessment Indonesian version (MoCA-Ina) which is a neuropsychological test. The plasma levels of beta amyloid, insulin, IDE and RAGE were measured by Elisa technique. Mean differences in the levels of biomarkers in both groups were analyzed using the Mann-Whitney U test and the association between two variables was analyzed using the chi-square test. Results: It was found that the plasma levels of insulin and beta amyloid 42 were lower in the group with cognitive impairment and there was an association between low plasma levels of insulin and beta amyloid 42 with the occurrence of cognitive impairment (p<0.05) Conclusion: There seems to be a correlation between plasma insulin level, beta amiloid and cognitive impairment in patients with diabetes mellitus..

Keywords: Biomarkers, cognitive disorders, diabetes mellitus

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Tip 2 Diabetes Mellitus’lu Hastalarda Görülen Biliþsel Bozukluk ile Beta Amiloid’in Plazma Düzeyleri, Ýnsülin-Parçalayan Enzim ve Ýleri Glikozilasyon Son Ürünlerinin Ýliþkisi

Yuliarni Syarita¹, Darwin Amir¹, Eva Decroli¹
Faculty of Medicine, Andalas University/DR M Djamil Hospital, Jalan Perintis Kemerdekaan No.94 Padang Timur, Padang, West Sumatra, Indonesia, Department of Neurology, Padang, Indonesia

Giriþ: Diabetes mellitus’lu hastalarda biliþsel bozukluk görülme olasýlýðý, bu hastalýðý bulunmayanlara göre daha yüksektir. Diabetes mellitus ile biliþsel bozukluk arasýndaki iliþkiyi açýklayabilecek insülin direnci, artmýþ amlioid oluþumu, insülin yýkan enzim (ÝYE) ve ileri glikozilasyon son ürünleri reseptörü (ÝGSÜ) gibi bir dizi neden bulunmaktadýr. Amaç: Bu çalýþmada amaç, diabetes mellituslu hastalarda baþlayan biliþsel bozukluk ile beta amiloid, insülin, ÝYE ve ÝGSÜ arasýndaki iliþkiyi araþtýrmaktýr. Yöntemler: Bu vaka kontrol çalýþmasýnda, biliþsel bozukluðu olan 30 hasta ile olmayan 30 olgu karþýlaþtýrýldý. Biliþsel bozukluk, bir nöropsikolojik test olan Montreal Biliþsel Deðerlendirme Testinin Endonezya sürüm’ü (MBDT-End) ile ölçüldü. Beta amiloid, insülin, ÝYE ve ÝGSÜ Elisa tekniði ile ölçüldü. Her iki gruptaki biobelirteç düzeyleri Mann- Whitney U testi, iki deðiþkenin arasýndaki iliþki ise Ki-Kare testi ile deðerlendirildi. Bulgular: Plazma insülin sevileri ile beta amiloid seviyeleri biliþsel bozukluðu olan hastalarda olmayanlara göre daha düþük bulundu. Plazma insülin seviyeleri ve beta amiloid ile biliþsel bozukluk arasýnda da baðýntý saptandý (p<0.05). Sonuç: Diabetes mellituslu hastalarda plazma insülin seviyeleri ve plazma amiloid düzeyleri ile biliþsel bozukluk arasýnda bir iliþki izlenmektedir.

Anahtar Kelimeler: Biobelirteçler, biliþsel bozukluklar, diabetes mellitus

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