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The Frequency and the Significance of TNF-α+CD19+ B-cell Population in Lymph Nodes of Patients with Squamous Cell Carcinoma of Head and Neck
Marzieh Norouzian1, Fereshteh Mehdipour2, Mohammad Javad Ashraf3, Bijan Khademi4, Abbas Ghaderi21Hormozgan University of Medical Sciences, School of Allied Medical Sciences, Department of Laboratory, Bandar Abbas, Iran2Shiraz University of Medical Sciences, School of Medicine, Shiraz Institute for Cancer Research, Shiraz, Iran
3Shiraz University of Medical Sciences, School of Medicine, Department of Oral Pathology, Shiraz, Iran
4Shiraz University of Medical Sciences, School of Medicine, Department of Otolaryngology, Shiraz, Iran
Objective: B-cells can contribute to the suppression or progression of the tumor growth through the secretion of various cytokines. In this study, the relevance of B-cell cytokine production to the outcome variables in patients having squamous cell carcinoma (SCC) of the head and neck was studied.
Materials and Methods: Thirty-six draining lymph nodes from untreated patients with tongue and larynx SCC were stimulated with CpG, recombinant CD40L and phorbol-12-myristate-13-acetate/ionomycin and analyzed by flow cytometry for the frequency of B-cell subsets based on interleukin (IL)-10 and tumor necrosis factor-alpha (TNF-α) cytokine production.
Results: It was indicated that patients with uninvolved lymph nodes and low stage of the disease had an increased percentage of TNF-α+CD19+ B-cells (p=0.006 and p=0.041, respectively), whereas the frequency of IL-10+CD19+ B-cells showed an increasing trend in tumor-draining lymph nodes (TDLNs) of patients with grade 2+3 compared to grade 1 (p=0.054).
Conclusion: Collectively, TNF-α-producing B-cells in TDLNs has been reported to be associated with good prognosticators in patients with SCC of head and neck that might have a positive role in immunity to SCC of head and neck.
Manuscript Language: English
