Turk J Immunol. 2021; 9(2): 80-85 | DOI: 10.5222/TJI.2021.03521

mRNA Expression Levels of NKp30, NKp46, NKG2D, Perforin and Granzyme in the Behçet’s Disease

Metin Yusuf Gelmez¹, Suzan Çýnar¹, Esin Aktaþ¹, Fatma Betül Öktelik¹, Goncagul Babuna-kobaner², Murat Erdugan³, Ahmet Gül³, Afet Akdað Köse², Günnur Deniz^1
1Istanbul University, Aziz Sancar Institute of Experimental Medicine, Department of Immunology, Istanbul, Turkey
²Istanbul University, Istanbul Faculty of Medicine, Department of Dermatology and Venereology, Istanbul, Turkey
³Istanbul University, Istanbul Faculty of Medicine, Department of Internal Medicine, Division of Rheumatology, Istanbul, Turkey

Objective: There are limited studies about NK cells that may have an immunoregulatory role in Behçet’s Disease. NK cell receptor, perforin and granzyme mRNA expressions were analyzed in both active and inactive BD patients.
Materials and Methods: NKp30, NKp46, NKG2D, perforin and granzyme mRNA expression were analyzed in 42 patients with Behçet’s disease and 14 controls by RT-PCR. CD3+ T, and NK cell subsets were analyzed by flow cytometry.
Results: NKp30, NKp46, NKG2D, perforin and granzyme expression levels between patients and healthy subjects did not show any significant differences. However, a positive correlation was detected between CD3-CD16+ CD56dim NK cell frequencies and NKG2D and NKp46 mRNA levels in active BD patients and between CD3+ T cell frequencies and NKp46 mRNA levels in inactive Behçet’s patients.
Conclusion: Although, a significant difference does not exist between NKp30, NKp46, NKG2D, perforin and granzyme mRNA expressions, further functional studies are necessary to rule out the potential role of these molecules in the pathogenesis of Behçet’s Disease.

Keywords: Behcet’s Disease, NK cell, NK receptor, NKp30, NKp46, NKG2D, perforin, granzyme

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Behçet Hastalarýnda NKp30, NKp46, NKG2D, Perforin ve Granzim mRNA Ekspresyon Düzeyleri

Metin Yusuf Gelmez¹, Suzan Çýnar¹, Esin Aktaþ¹, Fatma Betül Öktelik¹, Goncagul Babuna-kobaner², Murat Erdugan³, Ahmet Gül³, Afet Akdað Köse², Günnur Deniz^1
1Ýstanbul Üniversitesi, Aziz Sancar DETAE Ýmmünoloji A.D., Ýstanbul, Türkiye
²Ýstanbul Üniversitesi, Ýstanbul Týp Fakültesi, Dermatoloji A.D., Ýstanbul, Türkiye
³Ýstanbul Üniversitesi, Ýstanbul Týp Fakültesi, Ýç Hastalýklarý A.D., Romatoloji B.D., Ýstanbul, Türkiye

Amaç: Behçet Hastalýðýnda (BD) Doðal Katil (NK) hücrelerinin immünregülatör etkisine yönelik sýnýrlý sayýda çalýþma vardýr. Bu çalýþmada, aktif ve inaktif Behçet hastalarýnda NKp30, NKp46, NKG2D, perforin ve granzim mRNA ekspresyonlarý incelenmiþtir.
Gereç ve Yöntem: Çalýþmaya 42 Behçet hastasý ve 14 saðlýklý kontrol dahil edildi. NKp30, NKp46, NKG2D, perforin ve granzim mRNA ekspresyon düzeyleri RT-PCR ile, CD3+ T ve NK hücreleri akan hücre ölçer ile analiz edildi.
Bulgular: Hasta ve saðlýklý kontroller arasýnda NKp30, NKp46, NKG2D, perforin ve granzim mRNA ekspresyon düzeyleri açýsýndan anlamlý bir fark saptanmadý. Aktif hastalarda CD3-CD16+CD56dim NK hücre oranlarý ile NKG2D ve NKp46 mRNA ekspresyon seviyeleri arasýnda, inaktif hastalarda ise CD3+ T hücre oranlarý ile NKp46 mRNA ekspresyonlarý arasýnda pozitif korelasyon saptandý.
Sonuç: NKp30, NKp46, NKG2D, perforin ve granzim mRNA ekspresyonlarý açýsýndan anlamlý fark bulunmamakla birlikte bu moleküllerin Behçet patogenezindeki potansiyel rolünü dýþlamak için daha fazla fonksiyonel çalýþma gereklidir.

Anahtar Kelimeler: Behçet Hastalýðý, NK, NK reseptör, NKp30, NKp46, NKG2D, perforin, granzim

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