Turk J Immunol. 2018; 6(1): 1-7 | DOI: 10.25002/tji.2018.686

Improvement of Th17/Treg balance and Asthma Control Test score by Nigella sativa supplementation in asthmatic children: a new approach to managing

Wisnu Barlianto¹, Desy Wulandari¹, Maya Chusniyah¹, HMS Chandra Kusuma¹, Sumarno Reto Prawiro^2
1Department of Child Health, Faculty of Medicine, Brawijaya University, Saiful Anwar General Hospital, Indonesia.
²Department of Microbiology, Faculty of Medicine, Brawijaya University, Indonesia

Introduction: Th17 and Treg cells have important roles in the development and progression of the allergic diseases, such as asthma. Several studies reported that Nigella sativa could improve chronic airway inflammation, and inhibit airway remodeling by regulating the immune system. To examine the effect of Nigella sativa supplementation on Th17/ Treg cells, and asthma control test score in asthmatic children. Materials and Methods: Twenty-eight children with asthma meeting the inclusion criteria in Saiful Anwar Hospital, Indonesia, participated in this study. All subjects took the standard treatment based on asthma guidelines. Nigella sativa oil (NSO) is given as additional treatment at the dose of 15–30 mg/kg/day for eight weeks in a randomized, singleblind- controlled trial. Th17 and Treg in peripheral blood mononuclear cells were analyzed by flow cytometry tool. Improvement of asthma control was accessed by ACT (Asthma Control Test) Score. Results: There was a significant decrease of Th17, and an increase of Treg percentages in NSO treatment group. Th17/ Treg ratio was lower in NSO group compared to the standard treatment group (1.06±0.33 vs. 2.30±1.08, p=0.001). The ACT score improvement was significantly higher in NSO compared to that in the standard treatment group (3.71±1.634 vs. 1.86±0.864, p=0.033). However, there was no significant correlation between Th17/Treg ratio and ACT score in pre- and post-treatment (p=0.552, p=0.344 respectively). Conclusion: NSO supplementation improves Th17/Treg balance and clinical symptoms in asthmatic children.

Keywords: Treg, ACT score, asthma, Nigella sativa

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Astmalý Çocuklarda Nigella sativa Verilmesi ile Astma Kontrol Test Skoru ve Th17/Treg Dengesinde Düzelme: Astmanýn Tedavisinde Yeni Bir Yaklaþým

Wisnu Barlianto¹, Desy Wulandari¹, Maya Chusniyah¹, HMS Chandra Kusuma¹, Sumarno Reto Prawiro^2
1Department of Child Health, Faculty of Medicine, Brawijaya University, Saiful Anwar General Hospital, Indonesia.
²Department of Microbiology, Faculty of Medicine, Brawijaya University, Indonesia

Giriþ: Th17 ve Treg hücrelerinin, astma gibi allerjik hastalarýn ortaya çýkmasý ve ilerlemesinde önemli rolleri bulunur. Bir dizi çalýþma, Nigella sativanýn kronik hava yolu inflamasyonunu azalttýðýný ve baðýþýklýk sistemini düzenleyerek hava yollarýnýn etkilenmesini azalttýðýný göstermektedir. Gereçler ve Yöntemler: Çalýþmada, Endonezya Saiful Anwar Hastanesinde astma tanýsý koyulan 28 olgu irdelendi. Tüm hastalar, astma rehberine uygun olarak standart olarak tedavi aldý. Nigella sativa yaðý (NSY), randomize tek kör çalýþmada günde 15-30 mg/kg dozda 8 hafta boyunca verildi. Treg ve Th17 hücreleri periferik kan mononükleer hücrelerin içinde akan hücre ölçer ile sayýldý. Astmadaki düzelme, Astma Kontrol Testi (AKT) ile ölçüldü. Bulgular: NSY verilen grupta Th17 hücrelerinde anlamlý bir azalma, Treg hücrelerinin oranlarýnda da artýþ saptandý (1.06±0.33’e karþýn. 2.30±1.08, p=0.001). AKT skoru NSY verilen grupta, standart tedavi uygulanan hastalara göre anlamlý derecede daha yüksek idi (3.71±1.634’e karþýn 1.86±0.864, p=0.033). Ancak, tedavi öncesi ve sonrasýnda alýnan örneklerde Th17/Treg oranlarý ve AKT skorlarýnda bir deðiþim görülmedi (sýrasý ile; p=0.552, p=0344). Sonuç: NSY verilmesi Th17/Treg dengesini ve astmasý olan çocuklarda klinik bulgularý iyileþtirir.

Anahtar Kelimeler: Th17, Treg, AKT skoru, astma, Nigella sativa

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