Turk J Immunol. 2019; 7(1): 1-5 | DOI: 10.25002/tji.2019.782

Expression of Tumor Necrosis Factor-a and Interleukin-6 in Chronic Suppurative Otitis Media

Yan Edward¹, Jacky Munilson¹, Rossy Rosalinda¹, Hirowati Ali², Dolly Ýrfandy¹, Delva Swanda^1
1Department of ENT-HNS, Faculty of Medicine, Andalas University, West Sumatra, Indonesia
²Biomedical Laboratory, Faculty of Medicine, Andalas University, West Sumatra, Indonesia

Introduction: Chronic suppurative otitis media (CSOM) with cholesteatoma is decribed by keratin epithelial invasion in the middle ear and osteolysis in both of the ear bones and the temporal bone. Some inflammatory factors involved in bone resorption stimulation are Tumor Necrosis Factor Alpha (TNF-?) and Interleukin-6 (IL-6). The aim of this study is to determine the expression of TNF-? and IL-6 in CSOM patients with cholesteatoma. Materials and Methods: The design of this study is cross-sectional comparative analytic in 16 responders or CSOM patients with cholesteatoma and 16 samples of normal ear skin. The gene expressions of samples were examined through Real-Time Polymerase Chain Reaction (RT-PCR) method and were analyzed with statistics software (p<0.05). Results: The expression of TNF-? and IL-6 in CSOM patients with cholesteatoma were higher than their expression in normal ear skin. The expression of TNF-? was 0.1835±0.322 ng/ul in CSOM patients, while it is 0.005±0.006 ng/ ul in normal ear skin (p=0.043). In addition, the expression of IL-6 was 2.127±2.320 ng/ul in CSOM patients and 0.005±0.006 ng/ul in normal ear skin (p=0.010). Conclusion: The expression of TNF-? and IL-6 in CSOM patients with cholesteatoma are significantly different from patients with normal ear skin.

Keywords: Chronic suppurative otitis media, cholesteatoma, interleukin-6, real-time polymerase chain reaction, tumor necrosis factor-alpha

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Kronik Süpüratif Otitis Media’da Tümör Nekroz Faktör-a ve Ýnterlökin-6 Ýfadeleri

Yan Edward¹, Jacky Munilson¹, Rossy Rosalinda¹, Hirowati Ali², Dolly Ýrfandy¹, Delva Swanda^1
1Department of ENT-HNS, Faculty of Medicine, Andalas University, West Sumatra, Indonesia
²Biomedical Laboratory, Faculty of Medicine, Andalas University, West Sumatra, Indonesia

Giriþ: Kolesteatomlu Kronik süpüratif otitis media (KSOM), orta kulakta keratin epitel invazyonu ve kulak kemikleri ile birlikte temporal kemikte erime (osteoliz) ile tanýmlanmýþ bir hastalýktýr. Tümör Nekroz Edici Faktör-alfa (TNF-?) ve Ýnterlökin -6 (IL-6) kemik rezorpsiyonundan sorumlu enflamatuvar faktörler olarak tanýmlanmýþtýr. Bu çalýþmanýn amacý TNF-? ve IL-6 ifadesinin kolestetomlu KSOM’daki rolünü araþtýrmaktýr. Gereç ve Yöntemler: Çalýþma, 16 yanýtlý ya da kolesteatomlu hastanýn normal kulak cildinden alýnan örnekler ile çapraz karþýlaþtýrmalý olarak irdelenmesidir. Bulgular: Kolesteatomlu KSOM hastalarýnda TNF-? ve IL-6 ifadeleri, saðlýklý kulak cildi ile karþýlaþtýrýldýðýnda daha yüksek olarak bulundu. KSOM hastalarýnda TNF-? ifadesi 0.1835±0.322 iken normal ciltte bu ifade 0.005±0.006 (p=0.043) olarak saptandý. IL-6 ifadesi ise, hastalarda 2.127±2.320 iken normal kulak cildinde bu 0.005±0.006 olarak bulundu (p=0.01). Sonuç: Kolesteatomlu KSOM hastalarýnda TNF-? ve IL-6 düzeyleri normal cilde göre daha yüksektir.

Anahtar Kelimeler: Kronik süpüratif otitis media, kolesteatom, interlökin-6, gerçek zamanlý-polimeraz zincir reaksiyonu, tümör nekroz edici faktör-alfa

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