Abstract
Objectives:
This study aims to examine the effect of polyherbal erythropoiesis modulatory and stimulatory agent (EMSA) Eritin administration in modulating the activity of erythropoiesis on TER- 119+VLA-4+ cells and complement regulators TER-119+CD55+ and TER-119+CD59+ cells in Balb/c mice after total body irradiation (TBI) with a dose of 500 rad.
Materials and methods:
EMSA Eritin was administered orally in mice after exposure to TBI with a dose of 500 rad daily for two weeks with three different doses including a low dose (1.04 mg/g BW), normal dose (3.125 mg/g BW), and high dose (9.375 mg/g BW). Mice were treated with HEMAPO Epoetin alfa twice a week as positive control with a dose of 0.21 mg/g BW. On day 15, the bone marrow was isolated and the number of TER-119+VLA-4+, TER-119+CD55+ and TER-119+CD59+ cells were analyzed using flow cytometry. Examination showed marked reduction in the number of TER-119+VLA-4+ cells in the bone marrow of mice after TBI with dose of 500 rad compared to those of healthy controls. The number of TER-119+VLA-4+ cells in the irradiated mice was markedly increased with EMSA Eritin from low dose to high dose compared to mice with TBI and mice treated with Epoetin alfa.
Results:
The highest dose of EMSA Eritin was more effective to promote erythropoiesis than HEMAPO Epoetin alfa and the other dose of EMSA Eritin.
Conclusion:
Our study findings suggest that EMSA Eritin is a powerful medicinal herbal supplement which may serve as a protective agent to normalize homeostasis and erythropoiesis after irradiation with a dose of 500 rad.
Keywords:
Antioxidant, bone marrow, complement regulators, erythropoiesis, total body irradiationVOLUME
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ISSUE
Correspondence
Received
Accepted
Published
Suggested Citation
DOI
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