Abstract
Objective:
This study was conducted to determine the suppressor effect of propolis in controlling activated T cells, also T cells expressing TNF-a and IFN-y. Those molecules have an important role to generate acute inflammation in diabetes mellitus (DM) condition.
Material and Methods:
This study was done by in vivo experiment on streptozotocin-induced diabetic mice. Mice were classified into DM group (diabetic mice model without propolis ethanolic extract); propolis treatment group doses of 50, 100, and 200 mg/kg body weight; normal mice group. Effect of each treatment was observed after 14 days by flow cytometry analysis. The absolute numbers of CD4+CD62L-, CD4+TNF-a+, and CD4+IFN-y+ were assessed from splenic cells.
Results:
The data showed that administration of propolis ethanolic extract for 14 days significantly decreased the number of activated T cell. Propolis also decreased the expression of TNF-a and IFN-y by T cells. Administration of all doses of propolis caused decreased blood glucose level compared to baseline levels. Propolis administration at a dose of 100 and 200 mg/kg could suppress the expression of the pro-inflammatory cytokines.
Conclusion:
Propolis decreased the number of activated T cells expressing TNF-a and IFN-y. It also inhibited naive T cells to activate and prevented hyperglycemia.
VOLUME
,
ISSUE
Correspondence
Received
Accepted
Published
Suggested Citation
DOI
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