Ramya Raj
Devapriya Appukuttan
Sangeetha Subramanian
Prakash Psg
Jasmine Crena

Abstract

Objective:

Diabetes mellitus (DM) and periodontitis (PD) share similar pathophysiology including altered immune-inflammatory responses, and evidence implicates high mobility group box 1 (HMGB1)-receptor for advanced glycation end products (RAGE) axis in amplifying inflammation. Therefore, we aimed to investigate their expression in gingival tissues from individuals with Stage III/IV PD with and without type 2DM (T2DM).

Materials and Methods:

Thirty-three gingival tissue samples were taken from Group I: Systemically and periodontally healthy, Group II: Systemically healthy with PD and Group III: PD with T2DM. Tissue and gene expression were evaluated by immunohistochemistry and reverse transcription quantitative-polymerase chain reaction. The staining intensity distribution (SID) and the mean percentage of positive stained cells (MPPC) scores were reported.

Results:

When compared to group I, the gene expression of HMGB1 and RAGE were 2.85 and 2.50 times higher in PD+T2DM, respectively (p<0.001). MPPC and SID for HMGB1 and RAGE expression were high in Groups II and III, with increased expression in PD+T2DM. Furthermore, when compared to Group I, the expression was significantly higher (p<0.001). In the 33 samples, MPPC and SID scores for HMGB1 and RAGE in the epithelium and connective tissue significantly correlated with clinical parameters (p<0.001). With a diagnostic accuracy of 87.88% [95% confidence interval (CI): 72.67-95.18] and a positive and negative predictive value of 95% and 76.92%, respectively, HMGB1 was able to distinguish between periodontal health and disease. RAGE had a diagnostic accuracy of 93.94% (95% CI: 80.39-98.32) with a positive and negative predictive values of 91.67% and 100%, respectively.

Conclusion:

Increased HMGB1 and RAGE expression underscores their significance in amplifying inflammation in PD associated with T2DM. Furthermore, both can be used as diagnostic biomarkers.

Keywords:

High mobility group box 1, receptor for advanced glycated end products, periodontitis, diabetes mellitus, reverse transcription quantitative-polymerase chain reaction, immunohistochemistry

VOLUME

11

,

ISSUE

2
August 2023

Correspondence

Devapriya Appukuttan

Email

devapriyamds@gmail.com

Received

Accepted

Published

Suggested Citation

DOI

License

This work is licensed under the Creative Commons Attribution-NonCommercial-Non-Derivatives 4.0 International License (CC BY-NC-ND 4.0). License