Umut Can Küçüksezer
İlhan Tahralı
Suzan Adın Çınar
Esin Aktaş Çeti̇n
Bilun Gemicioğlu
Günnur Deniz

Abstract

Objectives:

This study aims to test tolerance-breaking molecules in an immunotherapy model.

Patients and methods:

Asthmatic patients (n=3) with successful subcutaneous immunotherapy were enrolled to study. Peripheral blood mononuclear cells isolated from heparinized blood were cultured in the absence or existence of immunotherapy allergens and control allergens, under interleukin (IL)-1β, IL-6, TLR4- and TLR8-ligand stimulation for five days. CD4+ T cell proliferation was investigated by flow cytometry, with CFSE dilution method.

Results:

All patients were CD4+ T cell unresponsive to immunotherapy allergens. This unresponsiveness was broken by IL-1β stimulation; however, it was maintained in the presence of certain control allergens.

Conclusion:

It is requisite to understand peripheral tolerance mechanisms and influential factors on these pathways to minimize the incidence of allergic diseases. It is possible to develop more effective and novel peripheral tolerance induction modalities by defining the factors which are capable of breaking peripheral tolerance.

Keywords:

Allergen, asthma, immunotherapy, inflammation, peripheral tolerance

VOLUME

1

,

ISSUE

3
December 2013

Correspondence

Günnur Deniz

Email

gdeniz@istanbul.edu.tr

Received

Accepted

Published

Suggested Citation

DOI

License

This work is licensed under the Creative Commons Attribution-NonCommercial-Non-Derivatives 4.0 International License (CC BY-NC-ND 4.0). License