Introduction: :

Chronic suppurative otitis media (CSOM) with cholesteatoma is decribed by keratin epithelial invasion in the middle ear and osteolysis in both of the ear bones and the temporal bone. Some inflammatory factors involved in bone resorption stimulation are Tumor Necrosis Factor Alpha (TNF-a) and Interleukin-6 (IL-6). The aim of this study is to determine the expression of TNF-a and IL-6 in CSOM patients with cholesteatoma.

Materials and Methods: :

The design of this study is cross-sectional comparative analytic in 16 responders or CSOM patients with cholesteatoma and 16 samples of normal ear skin. The gene expressions of samples were examined through Real-Time Polymerase Chain Reaction (RT-PCR) method and were analyzed with statistics software (p<0.05).

Results: :

The expression of TNF-a and IL-6 in CSOM patients with cholesteatoma were higher than their expression in normal ear skin. The expression of TNF-a was 0.1835±0.322 ng/ul in CSOM patients, while it is 0.005±0.006 ng/ ul in normal ear skin (p=0.043). In addition, the expression of IL-6 was 2.127±2.320 ng/ul in CSOM patients and 0.005±0.006 ng/ul in normal ear skin (p=0.010).

Conclusion: :

The expression of TNF-a and IL-6 in CSOM patients with cholesteatoma are significantly different from patients with normal ear skin.

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